Studies on nematocidal activity of chamaecrista nigricans, carica papaya and vernonia amygdalina extracts against ascaris suum on pigs in Serere district, Uganda.

dc.contributor.authorOcen, John Francis
dc.date.accessioned2026-09-24T09:22:07Z
dc.date.available2026-09-24T09:22:07Z
dc.date.issued2026
dc.descriptionUndergraduate research report
dc.description.abstractThis study investigated the acaricidal effects of Azadirachta Indica, Annonia squamosa and Solanum Indica plant extracts on Rephicephalus appendiculatus ticks in Kapelebyong District, Uganda. A cross-sectional descriptive survey was conducted among 30 livestock farmers in Acinga Sub-County to document indigenous tick-control practices, while an in vitro Adult Immersion Test (AIT) was used to evaluate Azadirachta indica, Annona squamosa and Solanum incanum. The extracts were tested at three recorded concentration levels (0.105, 0.125 and 0.145), with three replicates per treatment and 10 ticks per replicate. A standard acaricide (Amitraz) served as the positive control, while a negative control was included for comparison. Tick mortality was recorded at 0, 2, 5, 10 and 30 minutes. Survey data were summarized using frequencies and percentages, whereas laboratory data were analyzed using mean mortality, one-way analysis of variance (ANOVA) and Tukey's HSD test at a 5% level of significance. All 30 respondents had experienced tick infestation, cattle were identified as the most affected livestock by 96.7% of respondents, and 29 (96.7%) respondents reported using medicinal plants for tick control. Neem was the most frequently reported plant among medicinal-plant users (48.3%), followed by Sodom apple (31.0%) and custard apple (27.6%). At 30 minutes and concentration 0.145, Azadirachta indica and Solanum incanum each produced 100.0% mean mortality, while Annona squamosa produced 93.3%. Concentration significantly affected final mortality for Azadirachta indica (p = 0.011), Annona squamosa (p = 0.003) and Solanum incanum (p < 0.001). The highest-concentration plant extracts were not significantly different from the positive control at the final endpoint but were significantly different from the negative control. The study concludes that the selected plant extracts possess substantial in vitro acaricidal activity and merit further investigation through standardised extraction, toxicity assessment, in vivo testing and field evaluation.
dc.description.sponsorshipProf. Olila Deogracious ; Busitema University
dc.identifier.citationOcen, J. F. (2026). Studies on nematocidal activity of chamaecrista nigricans, carica papaya and vernonia amygdalina extracts against ascaris suum on pigs in Serere district, Uganda. [Unpublished undergraduate research report]. Busitema University.
dc.identifier.urihttps://bdears.busitema.ac.ug/handle/123456789/9646
dc.language.isoen
dc.publisherBusitema University
dc.titleStudies on nematocidal activity of chamaecrista nigricans, carica papaya and vernonia amygdalina extracts against ascaris suum on pigs in Serere district, Uganda.
dc.typeOther
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