Department of Chemistry

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Browsing Department of Chemistry by Author "Atenia Mathew"

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    Formulation of gelatin-chitosan hydrogel containing jatropha multifida leaf extract for treatment of wounds.
    (Busitema University, 2024) Atenia Mathew
    When the wound arises, there are some effects that will arise as the loss of all or part of the organ function, bacterial contamination. Wound healing healing can be with the use of traditional medicine. Some traditional remedies such as iodine plant (jatropha multifida) have been used to accelerate wound healing maximumly. Due to emergency of resistant bacterial strains and significant increase in side effects of currently available antibacterial drugs have made it urgent to develop research to identify new bio actives antibacterial compounds and innovative ways for drug delivery to enhance wound healing. This research aims to develop a novel hydrogel formulation that combines the beneficial properties of gelatin, chitosan and jatropha multifida leaf extract for effective wound healing. Hydrogel composed of gelatin, chitosan, and jatropha leaf extract can be one of the right candidates for wound dressing application which provides both an antibacterial and a proper wound drainage management property to promote faster healing. Herein, preparation of hydrogel has been conducted by the physical blending of the solution of gelatin, chitosan, and extract at 4O0C. Then, the mixture was cast to form hydrogel films by each 2-4 mm thickness and followed by drying at 37o C for 24 hours. The resulted hydrogels were characterized to confirm its potential as wound care dressing by gel fraction, swelling index, and anti-bacteria. The gel fraction of the hydrogel composed of 10 and 20 grams of gelatin (each with 0.5 grams of chitosan and 5ml grams of the extract) was respectively 68.86 % and 65.68%. The hydrogel, composed of 20 g of gelatin and 7.5 g of chitosan, has shown the highest water retention capacity (swelling index) by 400 %. However, the presence of the extract has slightly lowered both the gel fraction and swelling index of the resulted hydrogel. Jatropha multifida, a plant with known antimicrobial and anti-inflammatory properties, is being explored for its potential application in wound care. The combination of these components in a hydrogel formulation has the potential to create a synergistic effect that promotes wound healing by providing a moist environment, antimicrobial activity, and anti-inflammatory effects. The research involved extraction of the plant bioactive components, phytochemical analysis, antimicrobial investigation of the leaf extract of the plant and formulation of the hydrogel. Methods. The extract was obtained by maceration, phytochemical screening of jatropha multifida was carried out. The antimicrobial activity of the leaves was evaluated on the strains of s. aureus, and E. coli. Results: the presence of flavonoids, tannins, alkaloids, saponins was noted on the leaves of jatropha multifida. The ethanolic extract of the leaves did not show effective bacterial inhibition. Conclusion, this study holds promise for the development of an advanced wound dressing material that harnesses the therapeutic potential of natural ingredients combined with biocompatible polymers. The successful formulation of gelatin- chitosan based hydrogel loaded with j. Multifida leaf extract could offer a cost effective and sustainable approach to wound management with reducing reliance on synthetic materials and promoting the use of natural resources for medical applications.