The study deals with analysis of ascorbic acid content of mangoes grown from Mbale city. Iodometric titration method was used to determine the concentrations of Vitamin C in different mango. The results indicate that the Vitamin C concentrations in Alfonso and Palmer varieties was 62.172 mg/100g and 54.34 mg/100g respectively. The results show that there was difference between concentrations of Vitamin C within the varieties of mangoes. Precisely, considering mango varieties, Alfonso variety had the highest concentration of Vitamin C per 100 g than Palmer mango variety.